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Microbial diversity analysis of former salterns in southern Taiwan by 16S rRNA‐based methods by Chung‐Yi Wang; Chang‐Chai Ng; Tseng‐Wei Chen; Sz‐Jie Wu; Yuan‐Tay Shyu is a scholarly article available to read on EtoBox.

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## Abstract The microbiota diversity of the former salterns in southern Taiwan was investigated by denaturing gradient gel electrophoresis (DGGE) and fluorescence __in situ__ hybridization (FISH). Soil samples from three salterns were analyzed using DGGE and 16S rRNA from 502 colonies representing 5 archaea and 18 bacteria taxonomic groups. Each representative taxonomic group was further identified, whereas 8.7% of clones were unclassified microorganisms. __Chromohalobacter__, __Halomonas__ and __Virgibacillus__ are dominant in the Biemen saltern, Chiguensis saltern and Szutsau saltern, respectively. During FISH analysis, several taxonomic‐specific probes were used. The DAPI‐stained‐cell count in the Szutsao saltern had a higher number of microorganisms (4.58 × 10^7^ cell/cm^3^) than the other salterns. Archaea occupied 2.7–6.6% whereas bacteria accounted for 37.2–52.9% of total microbial population at the three sites. Among these three sampling sites, the Szutsao saltern had the highest diversity in halophilic microbial composition, as indicated by DGGE and FISH. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

Author

Chung‐Yi Wang; Chang‐Chai Ng; Tseng‐Wei Chen; Sz‐Jie Wu; Yuan‐Tay Shyu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Akademie Verlag; Wiley (ISSN 0233-111X)

Published

2007

Language

EN