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What is Formaldehyde RNA Gel Electrophoresis about?

This chapter describes a method for formaldehyde gel electrophoresis of total RNA. RNA has a tendency to form secondary structures that can impede separation during electrophoresis. This method uses formaldehyde as a denaturant in the agarose gel and adds formaldehyde and formamide to the RNA sample to aid in denaturation. The key steps are: 1) denaturing the RNA sample with formaldehyde and formamide, 2) preparing a 1% agarose gel with formaldehyde, 3) loading the denatured RNA samples, 4) running the gel

Author

Ghanshyam R Parmar

Language

EN