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Can I read Method for Rapid Determination of Hydroxyproline by High-performance Liquid Chromatography and Its Exploitation for the Study of Collagen Formation on EtoBox?
Method for Rapid Determination of Hydroxyproline by High-performance Liquid Chromatography and Its Exploitation for the Study of Collagen Formation by J. Macek; M. Adam is a Chemistry article available to read on EtoBox.
What is Method for Rapid Determination of Hydroxyproline by High-performance Liquid Chromatography and Its Exploitation for the Study of Collagen Formation about?
The accurate determination of hydroxyproline is needed for the estimation of total collagen in tissues. The usual methods are based on spectrophotometric procedures, e.g. the method of Stegeman [l] . This method lacks specificity and therefore it was modified by some authors [2-51, using extraction or ionexchange chromatography in order to remove interfering substances. With these modifications, however, the method became time-consuming. Since the linear range of the spectrophotometric methods is relatively narrow (l-10 I-(g of hydroxyproline), concentrated samples have to be diluted and measurements have to be repeated. For the determination of newly synthesized collagen, the conversion of radioactive proline into hydroxyproline is widely employed. Methods for the radiochemical assay of hydroxyproline [6--81 by silicic acid chromatography following oxidation and extraction into toluene are tedious and encounter serious losses of radioactivity. Dydek and Kehrer [9] have developed a highperformance liquid chromatographic (HPLC) procedure for separating hydroxyproline from proline. These amino acids were detected by calorimetry in the fractions, which is rather cumbersome. Recently,
Who reads Method for Rapid Determination of Hydroxyproline by High-performance Liquid Chromatography and Its Exploitation for the Study of Collagen Formation?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- J. Macek; M. Adam
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)
- Published
- 1986
- Language
- EN
- Field
- Chemistry (Physical Sciences)