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In vitro cartilage formation using TGF-β-immobilized magnetic beads and mesenchymal stem cell-magnetic bead complexes under magnetic field conditions by Mitsuru Motoyama; Masataka Deie; Atsushi Kanaya; Makoto Nishimori; Ayato Miyamoto; Shinobu Yanada; Nobuo Adachi; Mitsuo Ochi is a Engineering article available to read on EtoBox.
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## Abstract We evaluated the efficacy of transforming growth factor (TGF)‐β‐immobilized magnetic beads for chondrogenesis __in vitro__ using a mesenchymal stem cell (MSC) delivery system and an external magnetic force (EMF). MSCs isolated from the bone marrow of Sprague Dawley rats were mixed with carboxyl group‐combined magnetic beads (Ferri Sphere 100C®) coated with anti‐rat CD44 mouse monoclonal antibodies. TGF‐β3 (10 and 1 ng/mL) was attached magnetically to such other Ferri Sphere 100C® beads via an amide bond formed between a primary amino group on the TGF‐β3 and the carboxyl groups on the surface of the beads. MSC‐magnetic bead complexes were centrifuged to form a pellet and cultured in chondrogenic differentiation medium (CDM) supplemented with either 10 or 1 ng/mL TGF‐β‐immobilized magnetic beads (10 or 1 ng/mL TGF‐β‐immobilized magnetic bead groups) or in CDM supplemented with 1 or 10 ng/mL TGF‐β (1 or 10 ng/mL TGF‐β group). TGF‐β‐immobilized magnetic beads were gathered effectively under an EMF. Chondrogenesis was achieved from the MSC‐magnetic bead complexes in the presence of 1 ng/mL TGF‐β‐immobilized magnetic beads. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2
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- Author
- Mitsuru Motoyama; Masataka Deie; Atsushi Kanaya; Makoto Nishimori; Ayato Miyamoto; Shinobu Yanada; Nobuo Adachi; Mitsuo Ochi
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)
- Published
- 2009
- Language
- EN
- Field
- Engineering (Physical Sciences)