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Synthesis of aurone sulfonate derivatives: Evaluation of their cholinesterase inhibition, neuroprotective effects, and expr ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Chemistry 2 2.1.1 Synthesis of 6-hydroxybenzofuran-3(2H)-one 3 2.1.2 General synthesis of (Z)-2-benzylidene-6-hydroxybenzofuran-3(2H)-one derivatives 3 2.1.3 General synthesis of 6-benzenesulfonyl-benzofuran-3-one derivatives 3 3 Biological activity 3 3.1 In-vitro inhibition analysis on AChE 3 3.2 Molecular docking study 3 3.3 Cell line and cell culture 3 3.4 Cell viability assay 3 3.5 Protein carbonyl assay 5 3.6 Measurement of reactive oxygen species 5 3.7 Quantitative real-time reverse transcriptase (qRT)-PCR 5 3.8 Gene expression assays 5 3.9 Statistical analysis 5 4 Results 5 4.1 Chemistry 5 4.1.1 Synthesis of (Z)-2-(4-chlorobenzylidene)-3-oxo-2,3-dihydrobenzofuran-6-yl-benzenesulfonate (A0) 5 4.1.2 Synthesis of (Z)-2-(4)-chlorobenzylidene)-3-oxo-2,3-dihydrobenzofuran-6-yl-4‐methoxy benzenesulfonate (A1) 5 4.1.3 Synthesis of (Z)-2-(4-chlorobenzylidene)-3-oxo-2,3-dihydrobenzofuran-6-yl-4- nitrobenzenesulfonate (A2) 5 4.1.4 Synthesis of (Z)-2-(4-chlorobenzylidene)-3-oxo-2,3-dihydrobenzofuran-6-yl-4-chloro

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