Can I read Elevated expression of calcium-binding protein p9Ka is associated with increasing malignant characteristics of rat prostate carcinoma cells on EtoBox?

Elevated expression of calcium-binding protein p9Ka is associated with increasing malignant characteristics of rat prostate carcinoma cells by Youqiang Ke; Chun Jing; Roger Barraclough; Paul Smith; Michael P.A. Davies; Christopher S. Foster is a Medicine article available to read on EtoBox.

What is Elevated expression of calcium-binding protein p9Ka is associated with increasing malignant characteristics of rat prostate carcinoma cells about?

Northern and Western blotting techniques were used to study expression of the mRNA and corresponding protein product of the S100-related calcium-binding molecule p9Ka in 6 different metastatic cell lines of the Dunning R3327 rat prostate cancer model. In cells with the lowest metastatic capability (G cells), p9Ka mRNA was barely detectable. In 2 weakly metastatic cell lines (AT-1 and AT-2), p9Ka transcript amounts were, respectively, 6.29 6 0.74 and 5.55 6 1.11 times that detected in the G cells. In 3 highly metastatic cell lines (AT-3, MAT-LyLu and MAT-Lu), the amounts of p9Ka mRNA were, respectively, 12.85 6 2.82, 13.06 6 1.69 and 11.62 6 1.81 times that expressed in the G cells. Western blot analyses detected no p9Ka protein in the G cells. The amounts of p9Ka protein expressed by tumour cells of intermediate metastatic capability (AT-1 and AT-2) were 3.4 6 1.3 mg and 3.3 6 1.4 mg, respectively, per 1 3 10 6 cells. The amounts of p9Ka protein expressed by the tumour cells of highest metastatic capability (AT-3, MAT-LyLu and MAT-Lu) were 8.3 6 1.1 mg, 8.7 6 1.6 mg and 9.6 6 1.7 mg, respectively, per 1 3 10 6 cells. Our data reveal a direct association between the elevated express

Who reads Elevated expression of calcium-binding protein p9Ka is associated with increasing malignant characteristics of rat prostate carcinoma cells?

Author

Youqiang Ke; Chun Jing; Roger Barraclough; Paul Smith; Michael P.A. Davies; Christopher S. Foster

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1997

Language

EN

Field

Medicine (Health Sciences)