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Promoters Influence the Kinetics of Transgene Expression Following Adenovector Gene Delivery by Ping Chen; Jie Tian; Imre Kovesdi; Joseph T. Bruder is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract ## Background The kinetics of gene expression from adenovirus‐based delivery vectors will be an important variable influencing the efficacy and toxicity of these vectors. As different promoters have variable strengths and kinetic profiles, the optimal dose of a therapeutic transgene product over time may be achieved by varying the promoter. ## Methods We analyzed several viral and cellular promoters in the context of adenovector gene delivery in the mouse. The kinetics of transgene expression was evaluated following intramuscular and intravenous delivery. ## Results Transgene expression from the cytomegalovirus (CMV) promoter was rapidly down‐regulated in the tissues following intravenous administration of adenovectors. In contrast, transgene expression from the Rous sarcoma virus (RSV) promoter increased over time such that, at 3 weeks, expression was 10‐fold higher than that from the CMV promoter‐containing vector in all tissues. The kinetics of transgene expression from these vectors was similar when they were delivered via the intramuscular route in BALB/c, C57BL/6 and immunodeficient mice. Efficient repeat administration of an adenovirus vector, in the presence of

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Author

Ping Chen; Jie Tian; Imre Kovesdi; Joseph T. Bruder

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)