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This document outlines the procedures for eukaryotic target preparation for expression analysis using GeneChip probe arrays. It details the necessary reagents, materials, and step-by-step protocols for one-cycle and two-cycle target labeling assays, including RNA isolation, cDNA synthesis, and biotin labeling. The chapter aims to provide optimized methods for obtaining sufficient biotin-labeled cRNA for hybridization to GeneChip arrays.

Author

Rahil razak

Language

EN