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Quantitative real-time detection of parvovirus B19 DNA in plasma by Marco H.G.M. Koppelman; H. Theo M. Cuypers; Thomas Emrich; Hans L. Zaaijer is a Medicine article available to read on EtoBox.

What is Quantitative real-time detection of parvovirus B19 DNA in plasma about?

**BACKGROUND:** As of 2004, the European Pharmacopoeia demands that plasma pools for production of anti‐D immunoglobulin should not contain more than 10^4^ IU per mL of parvovirus B19 (B19V) DNA. Hence, before pooling, highly viremic donations have to be identified, and after pooling the level of B19V DNA must be determined. The performance of a new real‐time B19V DNA PCR test (Roche, Mannheim, Germany) was studied, using a DNA extractor (NucliSens, bioMerieux, Boxtel, the Netherlands) for isolation of nucleic acid, and using a DNA quantification test (LightCycler apparatus, Roche, Mannheim, Germany) for amplification and detection. **STUDY DESIGN AND METHODS:** Dilutions of the international B19V DNA standard and reference preparations were tested to determine the precision, linear range, and accuracy of the assay and to calculate the factor for conversion of B19V DNA copies to IUs. The internal control signals, invalid test results, and the effect of cryo‐poor plasma were studied as a measure for robustness. Routine performance was assessed by testing 164 manufacturing pools (not screened for B19V) and 1048 test pools of 480 donations each. **RESULTS:** The copies‐to‐IU conversio

Who reads Quantitative real-time detection of parvovirus B19 DNA in plasma?

Author

Marco H.G.M. Koppelman; H. Theo M. Cuypers; Thomas Emrich; Hans L. Zaaijer

Publisher

Elsevier Science; John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Elsevier; Elsevier BV; Wiley (ISSN 0372-1248)

Published

2004

Language

EN

Field

Medicine (Health Sciences)