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Functional characterization of prostaglandin F~2α~ receptor in the spinal cord for tactile pain (allodynia) by Tadatoshi Muratani; Mikio Nishizawa; Shinji Matsumura; Tamaki Mabuchi; Kohji Abe; Keiko Shimamoto; Toshiaki Minami; Seiji Ito is a Neuroscience article available to read on EtoBox.

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## Abstract Prostaglandin F~2α~ (PGF~2α~) binds to its receptor (FP) to increase the intracellular‐free calcium concentration ([Ca^2+^]i) by coupling of FP with G~q~ protein. Spinal intrathecal administration of PGF~2α~ to mouse induces touch‐evoked pain (mechanical allodynia), in which capsaicin‐insensitive primary afferent Aβ‐fibres and __N__‐methyl‐d‐aspartate receptor ɛ4 subunit are involved. FP in the spinal cord, however, was not well characterized. Here, we showed constitutive expression of FP mRNA in mouse spinal cord, and functionally characterized spinal FP‐expressing cells which were involved in PGF~2α~‐induced mechanical allodynia. The method for repetitive administration of oligodeoxyribonucleotides through tubing to conscious mice was established for mechanical allodynia evaluation. We identified an antisense oligodeoxyribonucleotide targeting FP mRNA, causing both disappearance of PGF~2α~‐induced mechanical allodynia and decrease of FP mRNA. With saline‐administered mice, PGF~2α~ rapidly increased [Ca^2+^]i of the cells in the deeper layer of the dorsal horn. In contrast, when the FP antisense oligodeoxyribonucleotide was repeatedly administered, the population of PG

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Author

Tadatoshi Muratani; Mikio Nishizawa; Shinji Matsumura; Tamaki Mabuchi; Kohji Abe; Keiko Shimamoto; Toshiaki Minami; Seiji Ito

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

2003

Language

EN

Field

Neuroscience (Life Sciences)