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MircoRNA-322-5p promotes lipopolysaccharide-induced acute kidney injury mouse models and mouse primary proximal renal tubular epithelial cell injury by regulating T-box transcription factor 21/mitogen-activated protein kinase/extracellular signal-related kinase axis 1 Introduction 2 Materials and methods 3 Cell culture 3 LPS-induced AKI mouse models inprotect protect unhbox voidb@x penalty @M {}vivo and inprotect protect unhbox voidb@x penalty @M {}vitro 3 Renal function test 3 Assessment of renal oxidative stress levels 3 Quantitative real-time PCR (qRT-PCR) 3 Vector construction and cell transfection 3 Western blot 3 Measurement of Caspase-3 and Caspase-9 activity 4 Dual luciferase reporter assay 4 RNA pulldown assay 4 Terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) 4 Flow cytometry apoptotic analysis 4 PAS (Periodic Acid-Schiff) staining 4 Histological analysis 4 Observation of mitochondrial morphology 5 U0126 treatment 5 Statistical analysis 5 Results 5 Establishment of LPS-induced AKI mice model and mouse renal tubular epithelial cell model 5 miR-322-5p promotes the apoptosis of AKI renal tubular epithelial cells 5 miR-322-5p promotes the apoptosis

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UND

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nonfiction