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An electrophysiological investigation of the characteristics and function of GABAA receptors on bovine adrenomedullary chromaffin cells by J. A. Peters; J. J. Lambert; G. A. Cottrell is a Medicine article available to read on EtoBox.

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The characteristics and function of T-aminobutyric acidA (GABAA) receptors expressed on bovine chromaffin cells in culture have been investigated using patch-clamp techniques. In voltage-clamped whole-cells, locally applied GABA (100 gM) evoked a transmembrane chloride current which demonstrated outward rectification. The amplitude of such currents was reversibly suppressed by the GABAA receptor antagonists bicuculline, picrotoxin and RU5135, and enhanced by the general anaesthetic propanidid. Glycine (100 gM) and baclofen (100 gM) were ineffective as agonists. In support of a physiological role for GABA in the adrenal medulla, the co-existence of GABAA and nicotinic acetylcholine (ACh) receptors was demonstrated on whole cells and outside-out membrane patches. Ionophoretically applied GABA reduced the amplitude of depolarization and action potential discharge occurring in response to locally applied ACh (100 gM), but had no effect upon the underlying ACh-induced current. In addition, an excitatory action of GABA was demonstrated by recording action potential waveforms in cell-attached patches. The results are discussed in the context of a GABA-ergic regulation of catecholamine sec

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Author

J. A. Peters; J. J. Lambert; G. A. Cottrell

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0031-6768)

Published

1989

Language

EN

Field

Medicine (Life Sciences)