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Staging of head and neck squamous cell carcinoma using theMET oncogene product as marker of tumor cells in lymph node metastases by Giorgio Cortesina; Tiziana Martone; Emanuela Galeazzi; Martina Olivero; Antonella De Stefani; Mario Bussi; Guido Valente; Paolo M. Comoglio; M. Flavia Di Renzo is a Medicine article available to read on EtoBox.
What is Staging of head and neck squamous cell carcinoma using theMET oncogene product as marker of tumor cells in lymph node metastases about?
In head and neck squamous cell carcinomas (HNSCC), metastasis to cervical lymph nodes is a major determinant of patient outcome. To detect metastases, we used the MET oncogene as marker, which encodes the receptor for hepatocyte growth factor/scatter factor, mediating epithelial cell motility and invasiveness. The MET gene is expressed in epithelia and over-expressed in carcinomas of specific histotypes, but not in lymphatic tissue. A total of 151 lymph nodes from 20 squamous cell carcinomas were studied with both in-depth histology and end-point and real-time quantitative RT-PCR. MET-encoded sequences were found in 61 of 151 nodes (40%), of which 24 (16%) were found metastatic by in-depth histopathology. Parallel routine histopathologic analysis of 654 lymph nodes from the same cases identified 36 metastases (5%). Real-time quantitative RT-PCR was used to measure MET gene-specific mRNA in normal tissues, primary tumors and lymphatic metastases and showed a 2-8fold increased expression in tumor cells which metastasize. RT-PCR for 3 cytokeratins expressed in HNSCC (K4, K10 and K13) proved to be less sensitive in detecting occult lymphatic metastases. Western blot analysis demonstrat
Who reads Staging of head and neck squamous cell carcinoma using theMET oncogene product as marker of tumor cells in lymph node metastases?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Giorgio Cortesina; Tiziana Martone; Emanuela Galeazzi; Martina Olivero; Antonella De Stefani; Mario Bussi; Guido Valente; Paolo M. Comoglio; M. Flavia Di Renzo
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2000
- Language
- EN
- Field
- Medicine (Health Sciences)