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Advanced PCR Techniques Overview by NT Ravindran is a document available to read on EtoBox.
What is Advanced PCR Techniques Overview about?
The document provides an overview of advanced PCR methods and applications. It discusses standard PCR using Taq polymerase for amplification of targets under 5 kb. It also describes high-fidelity PCR using Pfu polymerase for applications requiring cloning. Reverse transcriptase PCR is explained for analyzing gene expression from RNA templates. Real-time PCR allows for quantification of targets through fluorescent detection of amplification throughout the reaction. Advanced applications of PCR include long-r
- Author
- NT Ravindran
- Language
- EN