About this document
Cell Fusion and Somatic Hybridization by Rachelle Cajes is a document available to read on EtoBox.
Cell fusion involves fusing two different cells into a single unit containing genes from both original cells. The most widely used fusion agent is Sendai virus, which is inactivated using UV light or B-propiolactone. To isolate pure populations of human-mouse hybrid cells, a selection procedure uses HAT medium to kill parental and homokaryon cells, allowing only hybrid cells containing human and mouse genes to survive and grow. Chromosomes are often lost preferentially after fusion, with up to 95% of human
- Author
- Rachelle Cajes
- Language
- EN