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Electrochemical Detection of Triamterene in Human Urine Using Boron-doped Diamond Electrodes by Kanako Ishii; Genki Ogata; Yasuaki Einaga is a scholarly article available to read on EtoBox.

What is Electrochemical Detection of Triamterene in Human Urine Using Boron-doped Diamond Electrodes about?

Urine is one of the most used biological fluids for screening drug delivery and the resultant metabolites. In sports, the use of diuretics such as triamterene is considered a violation of anti-doping rules and is stipulated to be present at less than 79 nM in urine by the World Anti-Doping Agency (WADA). It is therefore important to develop effective rapid and low-cost tests for this diuretic. Here we apply electrochemical analysis using boron-doped diamond (BDD) electrodes, which have superior properties such as low background current, a wide potential window, and high resistance to deactivation. Since real urine samples show clear oxidation current peaks in the potential range more positive than 0.5 V (vs. Ag/AgCl) due to the presence of bio-components such as protein, uric acid, and ascorbic acid, to detect triamterene effectively, the electrochemical protocol was optimized towards a potential range where the other components have limited effect. Our results show that reduced triamterene exhibits an oxidation peak at 0.1 V (vs. Ag/AgCl) in 0.1 M phosphate buffer (PB) and at 0.2 V (vs. Ag/AgCl) in pooled human urine. The peak current value increased according to the triamterene c

Author

Kanako Ishii; Genki Ogata; Yasuaki Einaga

Publisher

Elsevier BV

Published

2022

Language

EN