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Recombination and lineage-specific mutations linked to the emergence of SARS-CoV-2 by Juan Ángel Patiño-Galindo; Ioan Filip; Ratul Chowdhury; Costas D. Maranas; Peter K. Sorger; Mohammed AlQuraishi; Raul Rabadan is a scholarly article available to read on EtoBox.

What is Recombination and lineage-specific mutations linked to the emergence of SARS-CoV-2 about?

The emergence of SARS-CoV-2 underscores the need to better understand the evolutionary processes that drive the emergence and adaptation of zoonotic viruses in humans. In the betacoronavirus genus, which also includes SARS-CoV and MERS-CoV, recombination frequently encompasses the Receptor Binding Domain (RBD) of the Spike protein, which, in turn, is responsible for viral binding to host cell receptors. Here, we find evidence of a recombination event in the RBD involving ancestral linages to both SARS-CoV and SARS-CoV-2. Although we cannot specify the recombinant nor the parental strains, likely due to the ancestry of the event and potential undersampling, our statistical analyses in the space of phylogenetic trees support such an ancestral recombination. Consequently, SARS-CoV and SARS-CoV-2 share an RBD sequence that includes two insertions (positions 432–436 and 460–472), as well as the variants 427N and 436Y. Both 427N and 436Y belong to a helix that interacts directly with the human ACE2 (hACE2) receptor. Reconstruction of ancestral states, combined with protein-binding affinity analyses using the physics-based trRosetta algorithm, reveal that the recombination event involving

Author

Juan Ángel Patiño-Galindo; Ioan Filip; Ratul Chowdhury; Costas D. Maranas; Peter K. Sorger; Mohammed AlQuraishi; Raul Rabadan

Publisher

Cold Spring Harbor Laboratory

Published

2020

Language

EN