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Noninvasive genotyping fetal Kell blood group (KEL1) using cell-free fetal DNA in maternal plasma by MALDI-TOF mass spectrometry by Ying Li; Kirstin Finning; Geoff Daniels; Sinuhe Hahn; Xiaoyan Zhong; Wolfgang Holzgreve is a Medicine article available to read on EtoBox.

What is Noninvasive genotyping fetal Kell blood group (KEL1) using cell-free fetal DNA in maternal plasma by MALDI-TOF mass spectrometry about?

## Abstract ## Background Alloimmunization against the fetal Kell (__KEL1__) blood group antigen is gaining importance relative to the Rhesus problem and is the second most important cause of hemolytic disease of the fetus and newborn. Molecular diagnosis for fetal __KEL1__, which currently involves invasive procedures, is routinely done for accessing whether a fetus is at risk. Here we developed a matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS)‐based single allele‐based extension reaction (SABER) to examine the fetal __KEL1__ gene from __KEL1__‐negative pregnant women using cell‐free fetal DNA in maternal plasma. ## Methods Thirty‐two maternal plasma samples taken at the second and third trimesters of gestation (median: 21.5 weeks) were examined with MALDI‐TOF MS‐based SABER. The results were confirmed by serological tests on cord blood or polymerase chain reaction (PCR) typing on amniocyte‐derived fetal DNA. ## Results We were able to detect the fetal __KEL1__ allele in 11 of the 13 __KEL1__‐positive samples. No false positive results were scored. The paternal __KEL1__ allele could be correctly determined in 94% of cases (30/32). ## Con

Who reads Noninvasive genotyping fetal Kell blood group (KEL1) using cell-free fetal DNA in maternal plasma by MALDI-TOF mass spectrometry?

Author

Ying Li; Kirstin Finning; Geoff Daniels; Sinuhe Hahn; Xiaoyan Zhong; Wolfgang Holzgreve

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0197-3851)

Published

2008

Language

EN

Field

Medicine (Health Sciences)