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Can I read DSC studies of the conformational stability of barstar wild-type on EtoBox?
DSC studies of the conformational stability of barstar wild-type by Arnulf Schöppe; Hans-Jürgen Hinz; Vishwas R. Agashe; S. Ramachandran; Jayant B. Udgaonkar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is DSC studies of the conformational stability of barstar wild-type about?
## Abstract The temperature induced unfolding of barstar wild‐type of bacillus amyloliquefaciens (90 residues) has been characterized by differential scanning microcalorimetry. The process has been found to be reversible in the pH range from 6.4 to 8.3 in the absence of oxygen. It has been clearly shown by a ratio of δ__H__~vH~/δ__H__~cal~ near 1 that denaturation follows a two‐state mechanism. For comparison, the C82A mutant was also studied. This mutant exhibits similar reversibility, but has a slightly lower transition temperature. The transition enthalpy of barstar wt (303 kJ mol^−1^) exceeds that of the C82A mutant (276 kJ mol^−1^) by approximately 10%. The heat capacity changes show a similar difference, δ__C__~p~ being 5.3 + 1 kJ mol^−1^ K^−1^ for the wild‐type and 3.6 δ 1 kJ mol^−1^ K^−1^ for the C82A mutant. The extrapolated stability parameters at 25 °C are δ__G__° = 23.5 + 2 kJ mol^−1^ for barstar wt and δ__G__^0^ = 25.5 + 2 kJ mol^−1^ for the C82A mutant.
Who reads DSC studies of the conformational stability of barstar wild-type?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Arnulf Schöppe; Hans-Jürgen Hinz; Vishwas R. Agashe; S. Ramachandran; Jayant B. Udgaonkar
- Publisher
- Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)
- Published
- 1997
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)