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Identification of protective outer membrane antigens of Brucella ovis by passive immunization of mice with monoclonal antibodies by Raúl A Bowden; Silvia M Estein; Michel S Zygmunt; Gérard Dubray; Axel Cloeckaert is a Medicine article available to read on EtoBox.

What is Identification of protective outer membrane antigens of Brucella ovis by passive immunization of mice with monoclonal antibodies about?

Outer membrane proteins (OMPs) and rough lipopolysaccharide (R-LPS), the main surface antigens of Brucella ovis, display surface-exposed epitopes. Mixtures of monoclonal antibodies (mAbs) to both antigens were previously shown to protect mice against a B. ovis challenge. To further identify the antigens involved, seven mAbs against Brucella OMPs (Omp10, Omp16, Omp19, Omp25, Omp31, Omp2b and Omp1) and three to R-LPS were tested for protection either individually or in combinations. Significant reduction in spleen infection in challenged mice, relative to controls, was used as the protection criteri. Controls included nonimmunized mice and mice given an irrelevant, anti-O-polysaccharide (OPS), mAb. For comparison, a group received a mouse serum containing antibodies to both OMPs and R-LPS; this serum was prepared by immunization with a B. ovis hot-saline extract which, as described previously, induces protective immunity in mice and rams. Significant protection was observed with both mAbs to OMPs and R-LPS. mAbs to Omp16, Omp19 and Omp31 afforded the highest protection and prevented the development of splenomegaly. The protective effect of mAb to Omp31 was not interfered with by nonp

Who reads Identification of protective outer membrane antigens of Brucella ovis by passive immunization of mice with monoclonal antibodies?

Author

Raúl A Bowden; Silvia M Estein; Michel S Zygmunt; Gérard Dubray; Axel Cloeckaert

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1286-4579)

Published

2000

Language

EN

Field

Medicine (Life Sciences)