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Identification and profiling of stable microRNAs in hemolymph of young and old Locusta migratoria fifth instars 1 Introduction 1 Materials & methods 2 Locusta migratoria rearing 2 Hemolymph collection and serum preparation 2 Synthetic oligoribonucleotide stability experiments 2 RNA extraction 2 cDNA synthesis and qRT-PCR 2 sRNA sequencing and data analysis 2 RNA interference experiments 3 In silico microRNA target predictions 3 Agomir experiments 3 Ecdysteroid measurements using an enzyme immunoassay 3 Data Availability Statement 3 Results 3 sRNAs are present in locust serum 3 miRNAs circulate in serum in a stable form 4 Temporal profile of ecdysteroids in hemolymph of fifth nymphal locusts 4 Differential analysis of extracellular sRNAs in hemolymph of young and old fifth nymphal instar locusts 4 Modulating the extracellular levels of microRNAs miR-13b and bantam affects the moulting process 5 Discussion 6 Declaration of Competing Interest 9 CRediT authorship contribution statement 9 Conflicts of interest and funding 9 Supplementary materials 9 Refereences 9
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Common subject areas: history, science, philosophy, social sciences.
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- UND
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- nonfiction