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Different Priming States of Synaptic Vesicles Underlie Distinct Release Probabilities at Hippocampal Excitatory Synapses by Mohammad Aldahabi; Flora Balint; Noemi Holderith; Andrea Lorincz; Maria Reva; Zoltan Nusser is a scholarly article available to read on EtoBox.

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## Summary A stunning example of synaptic diversity is the postsynaptic target cell-type-dependent difference in synaptic efficacy in cortical networks. Here, we show that CA1 pyramidal cell (PC) to fast spiking interneuron (FSIN) connections have 10-fold larger release probability (P~v~) than those on oriens lacunosum-moleculare (O-LM) interneurons. Freeze-fracture immunolabeling revealed that different nano-topologies and coupling distances between Ca^2+^ channels and release sites (RSs) are not responsible for the distinct P~v~. Although [Ca^2+^] transients are 40% larger in FSINs innervating boutons, when [Ca^2+^] entry is matched in the two bouton populations, EPSCs in O-LM cells are still 7-fold smaller. However, application of a phorbol ester analog resulted in a ∼2.5-fold larger augmentation at PC – O-LM compared to PC – FSIN synapses, suggesting incomplete docking or priming of vesicles. Similar densities of docked vesicles rule out distinct RS occupancies and demonstrate that incompletely primed, but docked, vesicles limit the output of PC – O-LM synapses.

Author

Mohammad Aldahabi; Flora Balint; Noemi Holderith; Andrea Lorincz; Maria Reva; Zoltan Nusser

Publisher

Elsevier BV

Published

2022

Language

EN