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Association of a DNA marker with Hessian fly resistance gene H9 in wheat by I. Dweikat; H. Ohm; S. Mackenzie; F. Patterson; S. Cambron; R. Ratcliffe is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Association of a DNA marker with Hessian fly resistance gene H9 in wheat about?

The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat (Triticum aestivum L.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25, have been identified in wheat. With near-isogenic wheat lines differing for the presence of an individual Hessian fly resistance gene, in conjunction with random amplified polymorphic DNA (RAPD) analysis and denaturing gradient-gel electrophoresis (DGGE), we have identified a DNA marker associated with the H9 resistance gene. The H9 gene confers resistance against biotype L of the Hessian fly, the most virulent biotype. The RAPD marker cosegregates with resistance in a segregating F2 population, remains associated with H9 resistance in a number of different T. aestivum and T. durum L. genetic backgrounds, and is readily detected by either DGGE or DNA gel-blot hybridization.

Who reads Association of a DNA marker with Hessian fly resistance gene H9 in wheat?

Author

I. Dweikat; H. Ohm; S. Mackenzie; F. Patterson; S. Cambron; R. Ratcliffe

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Springer Nature (ISSN 0040-5752)

Published

1994

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)