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A Calcium Phosphate-based Gene Delivery System by Huihua Fu; Yunhua Hu; Tim McNelis; Jeffrey O. Hollinger is a Engineering article available to read on EtoBox.

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## Abstract Although nonviral vectors have lower transfection efficiency than viral vectors, the excellent safety profile of nonviral vectors is appealing for gene therapy. An efficient, simple nonviral vector gene delivery system has been designed that includes plasmid DNA–calcium phosphate precipitates (pDNA‐CaP) and porous collagen spheres (CultispheresTM). The hypothesis for this study was the pDNA‐CaP would achieve efficient plasmid DNA transfection and the porous collagen spheres would provide a suitable delivery carrier system for three‐dimensional (3D) administration. To test the hypothesis, plasmid DNA including the LacZ reporter gene encoding β‐galactosidase was precipitated with CaP to form particles of compacted LacZ‐CaP and delivered directly or by CultispheresTM to cells __in vitro__. The transfection efficiency was determined by β‐galactosidase gene expression. Results indicated that pLacZ‐CaP promoted 25–84% of transfection efficiency in a broad cell line spectrum and in flexible experimental conditions. Maximum transfection efficiency was achieved by having mostly nano‐sized partles (50–200 nm in diameter) of pDNA‐CaP precipitates. Seeding density of 0.7–4 × 10^4^

Who reads A Calcium Phosphate-based Gene Delivery System?

Author

Huihua Fu; Yunhua Hu; Tim McNelis; Jeffrey O. Hollinger

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2005

Language

EN

Field

Engineering (Physical Sciences)