About this Biochemistry, Genetics and Molecular Biology article
Enzymatic α-Glucosaminylation of Maltooligosaccharides Catalyzed by Phosphorylase by Mutsuki Nawaji; Hironori Izawa; Yoshiro Kaneko; Jun-ichi Kadokawa is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
This paper describes phosphorylase-catalyzed enzymatic a-glucosaminylation for the direct incorporation of a 2-amino-2-deoxy-a-D-glucopyranose unit into maltooligosaccharides. When the reaction of 2amino-2-deoxy-a-D-glucopyranosyl 1-phosphate as the glycosyl donor with maltotetraose as a glycosyl acceptor was performed in the presence of phosphorylase, glucosaminylated oligosaccharides were produced, which were characterized by MALDI-TOF MS measurement after N-acetylation of the crude products. The N-acetylated derivative of the main product in this system was isolated by using HPLC, and its structure was confirmed by MS and 1 H NMR spectra. Furthermore, glucoamylase-catalyzed reaction of
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- Author
- Mutsuki Nawaji; Hironori Izawa; Yoshiro Kaneko; Jun-ichi Kadokawa
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)