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A Comparison of Liquid Chromatography, Capillary Electrophoresis, and Mass Spectrometry Methods to Determine Xyloglucan Structures in Black Currants by Hauke Hilz; Laura E. de Jong; Mirjam A. Kabel; Henk A. Schols; Alphons G.J. Voragen is a Chemistry article available to read on EtoBox.
What is A Comparison of Liquid Chromatography, Capillary Electrophoresis, and Mass Spectrometry Methods to Determine Xyloglucan Structures in Black Currants about?
Different separation (HPAEC, RP-HPLC, CE) and identification (MALDI-TOF-MS, ESI-MS(n)) techniques were compared to analyse oligosaccharides obtained after incubation of xyloglucan with endo-glucanase. It was possible to analyse xyloglucan oligosaccharides with each technique. Several techniques, including off line (HPAEC-MALDI-TOF-MS) or online (CE-ESI-MS(n), RP-HPLC-ESI-MS(n)) connection provided complementary information on xyloglucan structure. Online CE-MS and RP-HPLC-MS are described for the first time in xyloglucan analysis. Advantages and disadvantages of the techniques for different purposes such as structural characterisation of oligosaccharides or oligosaccharide profiling are discussed. Black currant xyloglucans had a rather simple XXXG-type structure with galactose and fucose containing side chains.
Who reads A Comparison of Liquid Chromatography, Capillary Electrophoresis, and Mass Spectrometry Methods to Determine Xyloglucan Structures in Black Currants?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Hauke Hilz; Laura E. de Jong; Mirjam A. Kabel; Henk A. Schols; Alphons G.J. Voragen
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2006
- Language
- EN
- Field
- Chemistry (Physical Sciences)