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Selective control of gene expression by CDK9 in human cells by Judit Garriga; Hongbo Xie; Zoran Obradovic; Xavier Graña is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract CDK9 associates with T‐type cyclins and positively regulates transcriptional elongation by phosphorylating RNA polymerase II (RNAPII) and negative elongation factors. However, it is unclear whether CDK9 is required for transcription of most genes by RNAPII or alternatively plays a role regulating the expression of restricted subsets of genes. We have investigated the direct effects of inhibiting cellular CDK9 activity in global gene expression in human cells by using a dominant‐negative form of CDK9 (dnCDK9). We have also compared direct inhibition of cellular CDK9 activity to pharmacological inhibition with flavopiridol (FVP), a CDK inhibitor that potently inhibits CDK9 and cellular transcription. Because of its presumed selectivity for CDK9, FVP has been previously used as a tool to infer the role of CDK9 on global gene expression. DNA microarray analyses described here show that inhibition of gene expression by FVP is consistent with global inhibition of transcription. However, specific inhibition of CDK9 activity with dnCDK9 leads to a distinctive pattern of changes in gene expression, with more genes being specifically upregulated (122) than downregulated (84). Ind

Who reads Selective control of gene expression by CDK9 in human cells?

Author

Judit Garriga; Hongbo Xie; Zoran Obradovic; Xavier Graña

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)