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Rapid Isolation of Biomarkers for Compound Specific Radiocarbon Dating Using High-performance Liquid Chromatography and Flow Injection Analysis–atmospheric Pressure Chemical Ionisation Mass Spectrometry by Rienk H Smittenberg; Ellen C Hopmans; Stefan Schouten; Jaap S Sinninghe Damsté is a Chemistry article available to read on EtoBox.

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Repeated semi-preparative normal-phase HPLC was performed to isolate selected biomarkers from sediment extracts for radiocarbon analysis. Flow injection analysis-mass spectrometry was used for rapid analysis of collected fractions to evaluate the separation procedure, taking only 1 min per fraction. In this way 100-1000 microg of glycerol dialkyl glycerol tetraethers, sterol fractions and chlorophyll-derived phytol were isolated from typically 100 g of marine sediment, i.e., in sufficient quantities for radiocarbon analysis, without significant carbon isotopic fractionation or contamination.

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Author

Rienk H Smittenberg; Ellen C Hopmans; Stefan Schouten; Jaap S Sinninghe Damsté

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2002

Language

EN

Field

Chemistry (Physical Sciences)