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Quercetin amelioratesAspergillus fumigatuskeratitis by inhibiting fungal growth, toll-like receptors and inflammatory cytokines by Jiao Yin; Xudong Peng; Jing Lin; Yingxue Zhang; Jie Zhang; Han Gao; Xue Tian; Ranran Zhang; Guiqiu Zhao is a Immunology and Microbiology article available to read on EtoBox.

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## Purpose: To investigate the antifungal and anti-inflammatory effects of quercetin on aspergillus fumigatus (a. fumigatus) keratitis. ## Methods: Human corneal epithelial cells (hcecs) and c57bl/6 mice were stimulated by a. fumigatus and treated with quercetin or dimethyl sulfoxide (dmso) after infection. in hcecs, minimum inhibitory concentration (mic) and cytotoxicity tests (cck-8) were used to detect the antifungal effect and cytotoxicity of quercetin. in mice with a. fumigatuskeratitis, clinical score, plate counting and hematoxylin-eosin (he) staining were performed to evaluate the effects of quercetin in vivo. myeloperoxidase (mpo) assay and immunofluorescence staining were applied to assess neutrophil recruitment and infiltration. real time pcr (rt-pcr), enzyme-linked immunosorbent assay (elisa) and western blot were used to detect the mrna and protein expressions of inflammatory mediators. ## Results: Compared with dmso control, quercetin (16-64 μm) significantly inhibited the growth of a. fumigatus in a concentration-dependent manner without affecting cell viability in hcecs. in corneas of mice with a. fumigatuskeratitis, quercetin decreased clinical score and fungal loa

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Author

Jiao Yin; Xudong Peng; Jing Lin; Yingxue Zhang; Jie Zhang; Han Gao; Xue Tian; Ranran Zhang; Guiqiu Zhao

Publisher

Elsevier BV

Published

2021

Language

EN

Field

Immunology and Microbiology (Life Sciences)