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Can I read Physiological Strains Induce Differentiation in Human Osteoblasts Cultured on Orthopaedic Biomaterial on EtoBox?
Physiological Strains Induce Differentiation in Human Osteoblasts Cultured on Orthopaedic Biomaterial by F. Di Palma; M. Douet; C. Boachon; A. Guignandon; S. Peyroche; B. Forest; C. Alexandre; A. Chamson; A. Rattner is a Engineering article available to read on EtoBox.
What is Physiological Strains Induce Differentiation in Human Osteoblasts Cultured on Orthopaedic Biomaterial about?
We have developed an in vitro mechanical stretching model of osteoblastic cells cultured on metallic biomaterials in order to study the effects of mechanical strain on osteointegration of orthopaedic implants. Titanium alloy discs coated with alumina or hydroxyapatite were used as substrates. Three Dynacell devices were especially designed to apply cyclic strains on rigid biomaterials. The regimen (600 mu epsilon strains, 0.25Hz) was defined on the basis of physiological data and estimated deformation on hip stem prostheses. The performances of these apparatus were reproducible and provided controlled deformations. Human osteosarcoma cell line MG-63, human osteoblasts obtained from primary cultures and ROS 17/2.8 rat osteosarcoma cells were used as cell models. Cell behaviour was assessed in terms of growth and alkaline phosphatase (ALP) activity by in situ assays for two regimens: 15-min deformations repeated three times a day to mimic rehabilitation exercises and 24-h continuous deformations. We demonstrated that continuous deformation did not affect the growth and ALP activity of MG-63 cells, in contrast with sequential deformations which had no effect on cell number, but which
Who reads Physiological Strains Induce Differentiation in Human Osteoblasts Cultured on Orthopaedic Biomaterial?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- F. Di Palma; M. Douet; C. Boachon; A. Guignandon; S. Peyroche; B. Forest; C. Alexandre; A. Chamson; A. Rattner
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)
- Published
- 2003
- Language
- EN
- Field
- Engineering (Physical Sciences)