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Can I read Application of recombinant DNA technologies to studies on chicken growth hormone on EtoBox?
Application of recombinant DNA technologies to studies on chicken growth hormone by Lawrence M. Souza; Thomas C. Boone; Doug Murdock; Keith Langley; Jette Wypych; Dennis Fenton; Steven Johnson; Por H. Lai; Richard Everett; Rou-Yin Hsu; Robert Bosselman is a Environmental Science article available to read on EtoBox.
What is Application of recombinant DNA technologies to studies on chicken growth hormone about?
Messenger RNA isolated from chicken pituitaries was used to construct a chicken pituitary cDNA library. A chicken growth hormone cDNA clone was isolated using 32P-labeled mammalian growth hormone cDNA probes. The amino acid sequence (derived from the DNA sequence) of the mature form of chicken growth hormone shows 77% homology with that of bovine growth hormone. The chicken growth hormone cDNA clone was used to generate a vector capable of producing chicken growth hormone in Escherichia coli. The recombinant E. coli-derived chicken growth hormone was similar to pituitary chicken growth hormone in several biochemical and immunological properties. The recombinant-derived hormone has been used to establish a sensitive radioimmunoassay for growth hormone determinations made from chicken sera. The chicken growth hormone gene has also been introduced into a retroviral vector capable of establishing productive infections of chicken cells both in in vitro and in vivo. The resulting infections are accompanied by the production of radioimmunoassay-detectable growth hormone. The concentrations of growth hormone in sera of Leghorn chickens infected with the recombinant retrovirus are three- to
Who reads Application of recombinant DNA technologies to studies on chicken growth hormone?
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Lawrence M. Souza; Thomas C. Boone; Doug Murdock; Keith Langley; Jette Wypych; Dennis Fenton; Steven Johnson; Por H. Lai; Richard Everett; Rou-Yin Hsu; Robert Bosselman
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-104X)
- Published
- 1984
- Language
- EN
- Field
- Environmental Science (Life Sciences)