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Modulation of invasive properties of murine squamous carcinoma cells by heterologous expression of cathepsin B and cystatin C by Sogué Coulibaly; Herwig Schwihla; Magnus Abrahamson; Adriana Albini; Christa Cerni; Jason L. Clark; Ken M. Ng; Nobuhiko Katunuma; Otto Schlappack; Josef Glössl; Lukas Mach is a Medicine article available to read on EtoBox.

What is Modulation of invasive properties of murine squamous carcinoma cells by heterologous expression of cathepsin B and cystatin C about?

Murine SCC-VII squamous carcinoma cells have the capacity to penetrate reconstituted basement membranes (Matrigel) in vitro. The invasion of Matrigel layers by SCC-VII cells was significantly reduced by E-64, a specific inhibitor of lysosomal cysteine proteinases. The cathepsin-B-selective E-64 derivative, CA-074, inhibited penetration of Matrigel by SCC-VII cells to the same extent, indicating a major role for this particular lysosomal enzyme in extracellular-matrix degradation during squamous-carcinoma-cell invasion. SCC-VII cells were stably transfected with a cDNA encoding human procathepsin B, in an attempt to modulate the invasive properties of the cell line. The transfected cells expressed the heterologous gene, secreted increased amounts of procathepsin B and displayed enhanced invasive potential. In vivo, the activity of cathepsin B is strictly regulated by endogenous inhibitors. SCC-VII cells were therefore also stably transfected with a cDNA encoding human cystatin C, the most potent cysteine-proteinase inhibitor in mammalian tissues. The expression of this transgene resulted in the production of active recombinant cystatin C and a pronounced reduction in Matrigel invasi

Who reads Modulation of invasive properties of murine squamous carcinoma cells by heterologous expression of cathepsin B and cystatin C?

Author

Sogué Coulibaly; Herwig Schwihla; Magnus Abrahamson; Adriana Albini; Christa Cerni; Jason L. Clark; Ken M. Ng; Nobuhiko Katunuma; Otto Schlappack; Josef Glössl; Lukas Mach

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1999

Language

EN

Field

Medicine (Health Sciences)