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Analysis of immunomodulating nitric oxide, iNOS and cytokines mRNA in mouse macrophages induced by microcystin-LR by Ting Chen; Xiangyi Zhao; Yang Liu; Qian Shi; Zichun Hua; Pingping Shen is a Environmental Science article available to read on EtoBox.

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Microcystins (MCs) are the toxic molecules produced by common cyanobacterium in freshwater blooms. Their toxicities have brought severe health issues to livestock and human being. Microcystin-LR (MC-LR) is one of the most toxic MCs. This paper presents the profile of the immunomodulation of MC-LR to BALB/c mice peritoneal macrophages. Macrophages were stimulated with 100 g/l lipopolysaccharide (LPS) and MC-LR at dose of 1, 10, 100, 1000 nmol/l, respectively, for 24 h. Nitric oxide (NO) production in cell culture supernatants was quantified by using Griess reagent method. Total RNA was extracted from incubated macrophages then the mRNA abundance of induced nitric oxide synthase (iNOS), IL-1␤, TNF-␣, GM-CSF, IFN-␥ was monitored by using reverse-transcriptional polymerase chain reaction (RT-PCR). The results demonstrated that NO production, mRNA levels of iNOS, IL-1␤, TNF-␣ were down regulated by MC-LR dose-dependently and mRNA levels of GM-CSF and IFN-␥ were also decreased but in dose-independent manner. Our results illustrated the involvement of NO production, iNOS and some cytokines in mice immune system in microcystin shock.

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Author

Ting Chen; Xiangyi Zhao; Yang Liu; Qian Shi; Zichun Hua; Pingping Shen

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0300-483X)

Published

2004

Language

EN

Field

Environmental Science (Life Sciences)