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Deletion of the betaine–GABA transporter (BGT1; slc6a12) gene does not affect seizure thresholds of adult mice by A.C. Lehre; N.M. Rowley; Y. Zhou; S. Holmseth; C. Guo; T. Holen; R. Hua; P. Laake; A.M. Olofsson; I. Poblete-Naredo; D.A. Rusakov; K.K. Madsen; R.P. Clausen; A. Schousboe; H.S. White; N.C. Danbolt is a scholarly article available to read on EtoBox.

What is Deletion of the betaine–GABA transporter (BGT1; slc6a12) gene does not affect seizure thresholds of adult mice about?

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain. Once released, it is removed from the extracellular space by cellular uptake catalyzed by GABA transporter proteins. Four GABA transporters (GAT1, GAT2, GAT3 and BGT1) have been identified. Inhibition of the GAT1 by the clinically available anti-epileptic drug tiagabine has been an effective strategy for the treatment of some patients with partial seizures. Recently, the investigational drug EF1502, which inhibits both GAT1 and BGT1, was found to exert an anti-convulsant action synergistic to that of tiagabine, supposedly due to inhibition of BGT1. The present study addresses the role of BGT1 in seizure control and the effect of EF1502 by developing and exploring a new mouse line lacking exons 3-5 of Abbreviations: EDTA, sodium ethylenediamine tetraacetate; EF1502, (N-[4,4-bis(3-methyl-2-thienyl)-3-butenyl]-4- (methylamino-4,5,6,7tetrahydrobenzo[d]isoxazol-3-ol; GABA, gamma-aminobutyric acid; KO, knockout with respect to BGT1 and implying homozygotous BGT1-fKO mice unless stated otherwise; NaPi, sodium phosphate buffer with pH 7.4; PTZ, pentylenetetrazol; SDS, sodium dodecyl sulfate; WT,

Author

A.C. Lehre; N.M. Rowley; Y. Zhou; S. Holmseth; C. Guo; T. Holen; R. Hua; P. Laake; A.M. Olofsson; I. Poblete-Naredo; D.A. Rusakov; K.K. Madsen; R.P. Clausen; A. Schousboe; H.S. White; N.C. Danbolt

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0920-1211)

Published

2011

Language

EN