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Can I read New role of the antidepressant imipramine as a Fascin1 inhibitor in colorectal cancer cells on EtoBox?
New role of the antidepressant imipramine as a Fascin1 inhibitor in colorectal cancer cells by Begoña Alburquerque-González; Manuel Bernabé-García; Silvia Montoro-García; Ángel Bernabé-García; Priscila Campioni Rodrigues; Javier Ruiz Sanz; Fernando F. López-Calderón; Irene Luque; Francisco José Nicolas; María Luisa Cayuela; Tuula Salo; Horacio Pérez-Sánchez; Pablo Conesa-Zamora is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is New role of the antidepressant imipramine as a Fascin1 inhibitor in colorectal cancer cells about?
Serrated adenocarcinoma (SAC) is more invasive, has worse outcomes than conventional colorectal carcinoma (CRC), and is characterized by frequent resistance to anti-epidermal growth factor receptor (EGFR) and overexpression of fascin1, a key protein in actin bundling that plays a causative role in tumor invasion and is overexpressed in different cancer types with poor prognosis. In silico screening of 9591 compounds, including 2037 approved by the Food and Drug Administration (FDA), was performed, and selected compounds were analyzed for their fascin1 binding affinity by differential scanning fluorescence. The results were compared with migrastatin as a typical fascin1 inhibitor. In silico screening and differential scanning fluorescence yielded the FDA-approved antidepressant imipramine as the most evident potential fascin1 blocker. Biophysical and different in vitro actin-bundling assays confirm this activity. Subsequent assays investigating lamellipodia formation and migration and invasion of colorectal cancer cells in vitro using 3D human tissue demonstrated anti-fascin1 and anti-invasive activities of imipramine. Furthermore, expression profiling suggests the activity of imipr
Who reads New role of the antidepressant imipramine as a Fascin1 inhibitor in colorectal cancer cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Begoña Alburquerque-González; Manuel Bernabé-García; Silvia Montoro-García; Ángel Bernabé-García; Priscila Campioni Rodrigues; Javier Ruiz Sanz; Fernando F. López-Calderón; Irene Luque; Francisco José Nicolas; María Luisa Cayuela; Tuula Salo; Horacio Pérez-Sánchez; Pablo Conesa-Zamora
- Publisher
- Korean Society of Medical Biochemistry and Molecular Biology; Springer Science and Business Media LLC; Nature Publishing Group; Seoul: Korean Society of Medical Biochemistry and Molecular Biology, 2009 (ISSN 1226-3613)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)