Opening book details…
Can I read Structure and function of CYP108D1 from Novosphingobium aromaticivorans DSM12444: an aromatic hydrocarbon-binding P450 enzyme on EtoBox?
Structure and function of CYP108D1 from Novosphingobium aromaticivorans DSM12444: an aromatic hydrocarbon-binding P450 enzyme by Stephen G. Bell; Wen Yang; Jake A. Yorke; Weihong Zhou; Hui Wang; Jeffrey Harmer; Rachel Copley; Aili Zhang; Ruimin Zhou; Mark Bartlam; Zihe Rao; Luet-Lok Wong is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Structure and function of CYP108D1 from Novosphingobium aromaticivorans DSM12444: an aromatic hydrocarbon-binding P450 enzyme about?
CYP108D1 from Novosphingobium aromaticivorans DSM12444 binds a range of aromatic hydrocarbons such as phenanthrene, biphenyl and phenylcyclohexane. Its structure, which is reported here at 2.2 A ̊resolution, is closely related to that of CYP108A1 (P450terp), an -terpineol-oxidizing enzyme. The compositions and structures of the active sites of these two enzymes are very similar; the most significant changes are the replacement of Glu77 and Thr103 in CYP108A1 by Thr79 and Val105 in CYP108D1. Other residue differences lead to a larger and more hydrophobic access channel in CYP108D1. These structural features are likely to account for the weaker -terpineol binding by CYP108D1 and, when combined with the presence of three hydrophobic phenylalanine residues in the active site, promote the binding of aromatic hydrocarbons. The haem-proximal surface of CYP108D1 shows a different charge distribution and topology to those of CYP101D1, CYP101A1 and CYP108A1, including a pronounced kink in the proximal loop of CYP108D1, which may result in poor complementarity with the [2Fe-2S] ferredoxins Arx, putidaredoxin and terpredoxin that are the respective redox partners of these three P450 enzymes.
Who reads Structure and function of CYP108D1 from Novosphingobium aromaticivorans DSM12444: an aromatic hydrocarbon-binding P450 enzyme?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Stephen G. Bell; Wen Yang; Jake A. Yorke; Weihong Zhou; Hui Wang; Jeffrey Harmer; Rachel Copley; Aili Zhang; Ruimin Zhou; Mark Bartlam; Zihe Rao; Luet-Lok Wong
- Publisher
- International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
- Published
- 2012
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)