About this document
Error Correction of Microchip Synthesized Genes Using Surveyor Nuclease by Cherry Kim is a document available to read on EtoBox.
This document discusses a method for error correction in microchip-synthesized genes using Surveyor nuclease, which identifies and excises mismatches in synthetic gene products. The process involves re-annealing the gene products, followed by a combination of mismatch-specific endonuclease and exonuclease activities to reduce errors significantly. The authors report achieving an error rate of approximately 1 in 8700 base pairs after two iterations of this error correction reaction.
- Author
- Cherry Kim
- Language
- EN