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Non-Steady State 13C-MFA Analysis by Mritunjoy Roy is a document available to read on EtoBox.

1. Isotopic steady state assumptions do not provide useful information for autotrophic organisms since their carbon source is inorganic carbon dioxide, leading to uniform labeling patterns at steady state. 2. Non-steady state 13C-MFA was developed to address this by estimating metabolic fluxes at metabolic pseudo steady state, assuming constant fluxes and pool sizes but using transient 13C labeling data and pool size measurements over time. 3. The parameter estimation involves numerically integrating i

Author

Mritunjoy Roy

Language

EN