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Bacterial components in the synovial tissue of patients with advanced rheumatoid arthritis or osteoarthritis: Analysis with gas chromatography‐mass spectrometry and pan‐bacterial polymerase chain reaction by Tong Chen; Marja Rimpiläinen; Reijo Luukkainen; Timö Möttönen; Tuomas Yli‐Jama; Jari Jalava; Olli Vainio; Paavo Toivanen is a Medicine article available to read on EtoBox.

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## Abstract ## Objective To study the presence of bacterial components in the synovial tissue (ST) of patients with advanced rheumatoid arthritis (RA). ## Methods ST was collected during joint surgery from 41 RA patients. Tissue from 39 patients with osteoarthritis (OA), 4 patients with undifferentiated inflammatory arthritis (UA), and 3 cases of accidental deaths served as controls. The pan‐bacterial polymerase chain reaction (PCR) with primers for the 23S ribosomal RNA (rRNA) and 16S rRNA genes was used to detect bacterial DNA. In addition, synovial fluid (SF) samples from patients with chlamydial reactive arthritis (ReA) were also examined by the same method. The positive controls, bacterial DNA or ST spiked with different living bacteria, were analyzed alongside clinical samples. Most of the ST samples were also analyzed by gas chromatography‐mass spectrometry (GC‐MS) for determining the presence of bacteria‐derived muramic acid. Strict precautions were followed in the clinics and the laboratory to prevent contamination. ## Results In GC‐MS analysis, muramic acid was observed in the ST from 4 of 35 RA patients and from 2 of 14 OA patients, but not in ST from 2 patients with UA

Who reads Bacterial components in the synovial tissue of patients with advanced rheumatoid arthritis or osteoarthritis: Analysis with gas chromatography‐mass spectrometry and pan‐bacterial polymerase chain reaction?

Author

Tong Chen; Marja Rimpiläinen; Reijo Luukkainen; Timö Möttönen; Tuomas Yli‐Jama; Jari Jalava; Olli Vainio; Paavo Toivanen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0004-3591)

Published

2003

Language

EN

Field

Medicine (Health Sciences)