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Whole-genome assembly of A02 bacteria involved in nitrogen fixation within cassava leaves by Danping Huang; Jie Ren; Xi Chen; Kashif Akhtar; Qiongyue Liang; Congyu Ye; Caiyi Xiong; Huahong He; Bing He is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Whole-genome assembly of A02 bacteria involved in nitrogen fixation within cassava leaves about?
## Abstract The endophytic nitrogen (N)-fixing bacterium A02 belongs to the genus Curtobacterium (Curtobacterium sp.) and is crucial for the N metabolism of cassava (Manihot esculenta Crantz). We isolated the A02 strain from cassava cultivar SC205 and used the 15N isotope dilution method to study the impacts of A02 on growth and accumulation of N in cassava seedlings. Furthermore, the whole genome was sequenced to determine the N-fixation mechanism of A02. Compared with low N control (T1), inoculation with the A02 strain (T2) showed the highest increase in leaf and root dry weight of cassava seedlings, and 120.3 nmol/(mL·h) was the highest nitrogenase activity recorded in leaves, which were considered the main site for colonization and N-fixation. The genome of A02 was 3,555,568 bp in size and contained a circular chromosome and a plasmid. Comparison with the genomes of other short bacilli revealed that strain A02 showed evolutionary proximity to the endophytic bacterium NS330 (Curtobacterium citreum) isolated from rice (Oryza sativa) in India. The genome of A02 contained 13 nitrogen fixation (nif) genes, including 4 nifB, 1 nifR3, 2 nifH, 1 nifU, 1 nifD, 1 nifK, 1 nifE, 1 nifN, an
Who reads Whole-genome assembly of A02 bacteria involved in nitrogen fixation within cassava leaves?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Danping Huang; Jie Ren; Xi Chen; Kashif Akhtar; Qiongyue Liang; Congyu Ye; Caiyi Xiong; Huahong He; Bing He
- Publisher
- Oxford University Press (OUP)
- Published
- 2023
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)