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Haploid induction via unpollinated ovule culture in Gerbera hybrida by Li, Fan; Cheng, Ying; Zhao, Xiaokun; Yu, Rongpei; Li, Huimin; Wang, Lihua; Li, Shenchong; Shan, Qinli is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Haploid induction via unpollinated ovule culture in Gerbera hybrida about?
## Abstract Ovule-derived haploid culture is an effective and important method for genetic study and plant breeding. Gerbera hybrida is a highly heterozygous species, and the lack of homozygous lines presents a challenge for molecular genetic research. Therefore, we performed haploid induction through unpollinated ovule culture and evaluated the effects of several important factors on this culturing procedure in G. hybrida, including genotype, low temperature, and the development seasons of the ovules. Among 45 G. hybrida cultivars analyzed, 29 cultivars exhibited adventitious bud induction via in vitro unpollinated ovule culture with significant different responses, indicating that the genotype of donor plants was a vital factor for inducibility. Four cultivars with significantly different induction rates, including one non-induced cultivar, were selected to analyze seasonal effects. Ovules extracted in the summer consistently had the highest induction rates, and even the non-induced cultivar included in the analysis could be induced at low levels when ovules from summer were used. Low temperature treatment could also promote adventitious bud induction, and in particular, a strong
Who reads Haploid induction via unpollinated ovule culture in Gerbera hybrida?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Li, Fan; Cheng, Ying; Zhao, Xiaokun; Yu, Rongpei; Li, Huimin; Wang, Lihua; Li, Shenchong; Shan, Qinli
- Publisher
- Springer Science and Business Media LLC; London: Nature Publishing Group; [London]: Springer Nature; Nature Publishing Group; Society for Mining, Metallurgy and Exploration Inc.; Research Square; Springer Nature; Island Press (ISSN 2045-2322)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)