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Induction of KDR expression in bovine arterial endothelial cells by thrombin: Involvement of nitric oxide by Jie Wang; Ikuo Morita; Mitsue Onodera; Sei-itsu Murota is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Thrombin, a multifunctional serine protease, is generated at the site with vascular injuries. It not only participates in the coagulation cascade, but also can induce a lot of events related to cell mitogenesis and migration. In this study, we investigated the effect of thrombin on endothelial cell proliferation induced by vascular endothelial growth factor (VEGF). Thrombin promoted proliferation of cultured bovine carotid endothelial cells in a time‐ and dose‐dependent manner. Moreover, it drastically enhanced the cell growth stimulated by VEGF. This stimulatory effect was reduced by inhibitors of either protein kinase C (PKC) or mitogen‐activated protein kinase kinase (MAPKK). Thrombin induced a significant increase in the level of mRNA of the kinase domain‐containing receptor (KDR), but not tms‐like tyrosine kinase (Flt‐1), in a time‐dependent manner, which reached the maximum after 24 h of stimulation. This increase coincides well with the KDR protein expression. The luciferase assay showed that thrombin induced an about 7.5‐fold increase in the KDR promoter activity compared with the control. This enhanced KDR promoter activity was also abolished by inhibitors of e

Who reads Induction of KDR expression in bovine arterial endothelial cells by thrombin: Involvement of nitric oxide?

Author

Jie Wang; Ikuo Morita; Mitsue Onodera; Sei-itsu Murota

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)