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Dissolved inorganic carbon utilization and the development of extracellular carbonic anhydrase by the marine diatom Phaeodactylum tricornutum by M. D. IGLESIAS-RODRIGUEZ; M. J. MERRETT is a Agricultural and Biological Sciences article available to read on EtoBox.

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## SUMMARY The presence of extracellular carbonic anhydrase (CA) in relation to medium composition was investigated using cultures of the marine diatom __Phaeodactylum tricornutum__ Bohlin. Large‐volume cultures, with low initial cell inocula were grown on ASP‐2 (no dissolved inorganic carbon (DIC), 550 μM NO~a~^−^), f/2 (2–0 mM DIC, 880μM NO~3~^−^) and modified f/2 (2.0 mM DIC, 20 μM NO~3~^−^ media. Cells growing on ASP‐2 showed extracellular CA in the early stages of growth, whereas extracellular CA was not detected until partial depletion of total DIC in the stationary phase for cultures on f/2 or modified f/2. Both HCO~3~^−^ and CO~2~ were important in carbon limitation, extracellular CA being present when the free‐CO~2~ concentration fell below 5 μM, but the HCO~3~^−^ concentration needed to be below 1 mM. When carbon‐replete cells were transferred to carbon‐limited conditions, extracellular CA was recorded within minutes, the process being light‐dependent and completely inhibited by 3,3,4‐dichlorophenyl‐l, 1‐dimethylurea (DCMU). The addition of DIC to carbon‐limited cells resulted in a rapid decrease in extracellular CA activity. The membrane‐impermeable inhibitor of carbonic

Who reads Dissolved inorganic carbon utilization and the development of extracellular carbonic anhydrase by the marine diatom Phaeodactylum tricornutum?

Author

M. D. IGLESIAS-RODRIGUEZ; M. J. MERRETT

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Cambridge University Press (CUP); Wiley (ISSN 0028-646X)

Published

1997

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)