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Expression of a cysteine proteinase inhibitor (oryzacystatin-I) in transgenic tobacco plants by Sameer A. Masoud; Lowell B. Johnson; Frank F. White; Gerald R. Reeck is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Expression of a cysteine proteinase inhibitor (oryzacystatin-I) in transgenic tobacco plants about?

Expression of cysteine proteinase inhibitors (cystatins) in tobacco or other plants has the potential for improving resistance against pathogens and insects that possess cysteine proteinases. A chimeric gene containing a cDNA clone of rice cystatin (oryzacystatin-I; OC-I), the cauliflower mosaic virus 35S promoter, and the nopaline synthase 3' region was introduced into tobacco plants by Agrobacterium tumefaciens. The presence of the chimeric gene in transgenic plants was detected by a polymerase chain reaction-amplified assay, and transcriptional activity was shown by RNA blot analysis. Heated extracts from transgenic tobacco plants, as well as from progeny which were obtained by selfing a primary transformant, contained protein bands that corresponded in molecular mass to OC-I and reacted with antibodies raised against rOC, a recombinant OC-I protein produced by Escherichia coli. Similar bands were absent in extracts from untransformed control plants. OC-I levels reached 0.5~/o and 0.6~o of the total soluble proteins in leaves and roots, respectively, of some progeny. On a fresh weight basis, the OC-I content was higher in leaves (50 #g/g) than in roots (30 #g/g). OC-I was partia

Who reads Expression of a cysteine proteinase inhibitor (oryzacystatin-I) in transgenic tobacco plants?

Author

Sameer A. Masoud; Lowell B. Johnson; Frank F. White; Gerald R. Reeck

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1993

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)