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Black rice anthocyanins alleviate hyperuricemia in mice: Possible inhibitory effects on xanthine oxidase activity by cyanid ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials and reagents 2 2.2 Extraction and purification of anthocyanins from black rice 2 2.3 Inhibition of enzyme activity in vitro 2 2.4 Kinetic analysis of the type of enzymatic inhibition of XO 2 2.5 Fluorescence quenching analysis 3 2.6 Circular dichroism 3 2.7 Hypouricemic effect of black rice anthocyanins in mice 3 2.7.1 Animals preparation 3 2.7.2 Potassium oxonate induced hyperuricemia animal model 3 2.7.3 Extraction of blood, liver and kidney tissues and determination of organ index in mice 3 2.7.4 Determination of relevant indicators in mouse serum and liver homogenates 3 2.8 Statistical analysis 3 3 Results and discussion 3 3.1 In vitro inhibition of XO activity by purified fractions and crude extracts of black rice anthocyanins 3 3.2 Kinetic analysis of the type of enzymatic inhibition of XO 5 3.3 Fluorescence quenching analysis of the fraction 2 5 3.4 Circular dichroism of the interaction between XO and fraction 2 6 3.5 Effects of fraction 2 and crude extracts on hyperuricemic mice 6 3.5.1 Eva

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