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Integration of textural and spectral features of Raman hyperspectral imaging for quantitative determination of a single mai ... 1 1 Introduction 1 2 Methods 2 2.1 Sample preparation 2 2.2 Raman hyperspectral data collection and extraction 2 2.3 Detection of quantity of maize fungal spore 2 2.4 Hyperspectral imaging and textural feature extraction 3 2.5 Spectral and textural data preprocessing 3 2.6 Chemometric modeling methods 4 2.7 Evaluation indexes of model accuracy 4 3 Results and discussion 4 3.1 The mildew process of maize 4 3.2 Raman spectral preprocessing 5 3.3 Quantitative determination of fungal spores in a single maize based on Raman spectral feature 5 3.4 Raman hyperspectral textural feature 6 3.5 Quantitative determination of fungal spores in a single maize kernel based on Raman spectral and textural feature 6 3.6 Effective variables selection 6 3.7 Independent validation of the optimal model 6 3.8 Visualization of maize mildew process 6 4 Conclusion 8 CRediT authorship contribution statement 8 Declaration of Competing Interest 8 Acknowledgment 8 Appendix A Supplementary data 8 References 8

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nonfiction