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Chromosomal constraints in Gram‐positive bacteria revealed by artificial inversions by N. Campo; M. J. Dias; M. ‐L. Daveran‐Mingot; P. Ritzenthaler; P. Le Bourgeois is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Chromosomal constraints in Gram‐positive bacteria revealed by artificial inversions about?
## Summary We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram‐positive bacterium __Lactococcus lactis__. Large inversions, 80–1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructed using the site‐specific Cre‐__loxP__ recombination system. These inversions were all mechanistically feasible and fell into various classes according to stability and effect on cell fitness. The __L. lactis__ chromosome supports only to some extent unbalance in length of its replication arms. The location of detrimental inversions allowed identification of two constrained chromosomal regions: a large domain covering one fifth of the genome that encompasses the origin of replication (Ori domain), and a smaller domain located at the opposite of the chromosome (Ter domain).
Who reads Chromosomal constraints in Gram‐positive bacteria revealed by artificial inversions?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- N. Campo; M. J. Dias; M. ‐L. Daveran‐Mingot; P. Ritzenthaler; P. Le Bourgeois
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)