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Substrate adhesiveness and experimental metastatic potential of rat ascites hepatoma AH7974-derived variant sublines by Takanori Kawaguchi; Seiji Igarashi; Hironao Wakabayashi; Sachihiko Yokoya; Kiyoshi Fukui is a Medicine article available to read on EtoBox.
What is Substrate adhesiveness and experimental metastatic potential of rat ascites hepatoma AH7974-derived variant sublines about?
Cells of an adherent subline (74AD, adhesion greater than 95%) and a floating subline (74FL, adhesion less than 1%) of rat ascites hepatoma AH7974 produced substrates containing fibronectin (FN), laminin (LN) and type IV collagen (CL-IV), with 74AD cells producing higher levels of each component. 74AD cells possessed high adhesion affinities to LN and CL-IV substrates. By contrast, 74FL cells hardly adhered to these purified attachment proteins. The difference in adhesion between the two lines in vitro tended to increase on incubation of the cells in medium containing fetal bovine serum. However, 74FL and 74AD cells adhered avidly to the extracellular matrix (ECM) of vascular endothelial cells. Although the cell-ECM adhesion apparently was not inhibited by pretreatment of the ECM with anti-FN, anti-LN and anti-CL-IV antibodies, the 74FL cell-ECM adhesion was inhibited considerably by pretreatment of the ECM with a mixture of these antibodies, especially with a combination of anti-FN and anti-LN antibodies. The lung-colonizing potential of 74FL cells was greater than that of 74AD cells, but the liver-colonizing potential of 74FL cells was less than that of 74AD cells. These results
Who reads Substrate adhesiveness and experimental metastatic potential of rat ascites hepatoma AH7974-derived variant sublines?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Takanori Kawaguchi; Seiji Igarashi; Hironao Wakabayashi; Sachihiko Yokoya; Kiyoshi Fukui
- Publisher
- Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0262-0898)
- Published
- 1992
- Language
- EN
- Field
- Medicine (Health Sciences)