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Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy by Hsiang-Hsin Chen; Chia-Chi Chien; Cyril Petibois; Cheng-Liang Wang; Yong S Chu; Sheng-Feng Lai; Tzu-En Hua; Yi-Yun Chen; Xiaoqing Cai; Ivan M Kempson; Yeukuang Hwu; Giorgio Margaritondo is a Engineering article available to read on EtoBox.

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## Background Quantitative analysis of nanoparticle uptake at the cellular level is critical to nanomedicine procedures. In particular, it is required for a realistic evaluation of their effects. Unfortunately, quantitative measurements of nanoparticle uptake still pose a formidable technical challenge. We present here a method to tackle this problem and analyze the number of metal nanoparticles present in different types of cells. The method relies on high-lateral-resolution (better than 30 nm) transmission x-ray microimages with both absorption contrast and phase contrast -- including two-dimensional (2D) projection images and three-dimensional (3D) tomographic reconstructions that directly show the nanoparticles. ## Results Practical tests were successfully conducted on bare and polyethylene glycol (PEG) coated gold nanoparticles obtained by x-ray irradiation. Using two different cell lines, EMT and HeLa, we obtained the number of nanoparticle clusters uptaken by each cell and the cluster size. Furthermore, the analysis revealed interesting differences between 2D and 3D cultured cells as well as between 2D and 3D data for the same 3D specimen. ## Conclusions We demonstrated the

Who reads Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy?

Author

Hsiang-Hsin Chen; Chia-Chi Chien; Cyril Petibois; Cheng-Liang Wang; Yong S Chu; Sheng-Feng Lai; Tzu-En Hua; Yi-Yun Chen; Xiaoqing Cai; Ivan M Kempson; Yeukuang Hwu; Giorgio Margaritondo

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2003-; Springer Science and Business Media LLC (ISSN 1477-3155)

Published

2011

Language

EN

Field

Engineering (Physical Sciences)