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Can I read Brd4 Is Required for Chondrocyte Differentiation and Endochondral Ossification on EtoBox?
Brd4 Is Required for Chondrocyte Differentiation and Endochondral Ossification by Christopher R. Paradise; M. Lizeth Galvan; Oksana Pichurin; Sofia Jerez; Eva Kubrova; S. Sharare Dehghani; Margarita E. Carrasco; Roman Thaler; A. Noelle Larson; Andre J. van Wijnen; Amel Dudakovic is a Medicine article available to read on EtoBox.
What is Brd4 Is Required for Chondrocyte Differentiation and Endochondral Ossification about?
Differentiation of multi-potent mesenchymal stromal cells (MSCs) is directed by the activities of lineage-specific transcription factors and co-factors. A subset of these proteins controls the accessibility of chromatin by recruiting histone acetyl transferases or deacetylases that regulate acetylation of the N-termini of H3 and H4 histone proteins. Bromodomain (BRD) proteins recognize these acetylation marks and recruit the RNA pol II containing transcriptional machinery. Our previous studies have shown that Brd4 is required for osteoblast differentiation in vitro. Here, we investigated the role of Brd4 on endochondral ossification in C57BL/6 mice and chondrogenic differentiation in cell culture models. Conditional loss of Brd4 in the mesenchyme (Brd4 cKO, Brd4: Prrx1-Cre) yields smaller mice that exhibit alteration in endochondral ossification. Importantly, abnormal growth plate morphology and delayed long bone formation is observed in juvenile Brd4 cKO mice. One week old Brd4 cKO mice have reduced proliferative and hypertrophic zones within the physis and exhibit a delay in the formation of the secondary ossification center. At the cellular level, Brd4 function is required for c
Who reads Brd4 Is Required for Chondrocyte Differentiation and Endochondral Ossification?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Christopher R. Paradise; M. Lizeth Galvan; Oksana Pichurin; Sofia Jerez; Eva Kubrova; S. Sharare Dehghani; Margarita E. Carrasco; Roman Thaler; A. Noelle Larson; Andre J. van Wijnen; Amel Dudakovic
- Published
- 2021
- Language
- EN
- Field
- Medicine (Health Sciences)